Introduction
The discovery of induced pluripotent stem cells (iPSC) and the direct conversion approach opened an attractive avenue that resolves these problems. The direct conversion approach and the generation of iPSCs provide an invaluable resource of cells for disease modeling, drug screening, and patient-specific cell-based therapy.
However, in contrast to ESCs, the quality of iPSCs varies widely between different colonies, and a large proportion of these colonies is of low developmental potential. In addition, iPSCs are more prone to malignant transformation.
Features / strengths
This disclosure provides isolation and preparation of human trophoblast stem cells which are obtained from a term placenta. This disclosure also provides a method for obtaining human trophoblast stem cells comprising obtaining a term placenta;
obtaining human placental cytotrophoblasts from the term placenta; manipulating the expression of GCM1 (glial cells missing 1) and ΔNp63α in the obtained human placental cytotrophoblasts. This disclosure also provides a composition for therapy
comprising isolation and preparation of human trophoblast stem cells and a buffer solution.
Specification in detail
Under development
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