Introduction
1. The ZNA synthetically modified probe Probe can effectively increase the Tm value and accurately predict the Tm value.
2. High sensitivity, can detect fluorescent signals earlier
3. Highly specific
4. Identify target sequences more quickly
5. Sequence design is easy and can be used as primer and probe
6. Suitable for PCR/qPCR/SNP, microarray, etc.
Features / strengths
Zip Nucleic Acid (ZNA) is a new type of nucleotide modification. ZNA binding is to add a positively charged spermine unit (also called Z unit) to the outside of the DNA sequence. The positively charged structure helps reduce the negative repulsion between ZNA and the complementary sequence, embeds the complementary sequence and forms It looks like a zipper structure, as shown in the action mechanism diagram below, so it is named Zip Nucleic Acid.
By reducing the repulsion between nucleotides, ZNA improves the hybridization ability and Tm value with the target sequence, reducing the probability of mismatch discrimination. Since the chemical structure of the nucleotide sequence of ZNA is still in the form of DNA, it can be used as a PCR The primer or probe in .
The factors that affect the Tm value of ZNA are: the length of the nucleotide sequence. The longer the sequence, the higher the Tm value. The other is the number of positively charged groups. The greater the number, the higher the Tm value. Based on the nucleotide sequence and the number of positively charged groups, the Tm value can be accurately predicted by calculating with the following formula.
Tm (ZNA™) = Tm (DNA) + 36z/(N-3.2)
(Z: number of positively charged spermine groups (Z unit) N: number of nucleotides)
Specification in detail
Product Information
Please email us for detailed specifications.
