Introduction
Sahara Hot Start PCR Master Mix is a high-efficiency 2X Taq mix ideal for endpoint PCR, sequencing, and cloning applications, as well as the quantitative amplification of singleplex qPCR targets using probes.
Features / strengths
◎Extreme thermal stability: withstands three months at 25 ºC with no impact on efficiency
◎Aptamer-based hot start system reduces non-specific amplification and primer dimers
◎Overcomes inhibition in crude extracts and environmental samples
◎Amplifies up to 4 kb from genomic DNA, and GC-rich targets with up to 70% GC-content
◎License-free for commercial or diagnostic use
Specification in detail
Hot Start Mechanism
Aptamer
Exonuclease Activity
5' → 3'
Supported Probes
TaqMan/Hydrolysis, Molecular Beacon, Scorpions
Supported Templates
Genomic DNA, cDNA, Plasmid DNA
Recommended Reaction Volume
10 – 50 µL
