Introduction
Traditional SYBR Green not effective? Want to get more sensitive Melt and HRM analysis results?
Come and try CHAI Green & Sahara Multiplex qPCR Master Mix!
Performs better than traditional SYBR Green and does not inhibit PCR reactions
Higher sensitivity Melt and HRM analysis available
GC Rich reaches 70% and still performs well
The reagent contains special Enhance, which can effectively inhibit the PCR Inhibitor in the DNA extraction solution.
Multiplex PCR (up to 4-plex) possible
High thermal stability, can be stored at 25 ºC for 3 months without affecting performance (50 ºC for 8 days, or 25 ºC for 3 months)
Features / strengths
◎Multiplex up to four Real-Time PCR reactions with no effect on Cq’s
◎Extreme thermal stability: withstands three months at 25 ºC with no impact on efficiency
◎Overcomes inhibition in crude extracts and environmental samples
◎Universal mix runs fast protocols, amplifies GC-rich targets, and incorporates aptamer-based hot start and dUTP carry-over prevention systems
◎License-free for commercial or diagnostic use
Specification in detail
Hot Start Mechanism
Aptamer
Multiplex Targets
Up to 4
dUTP Carryover Prevention
Yes
Exonuclease Activity
5' → 3'
Supported Templates
Genomic DNA, cDNA, Plasmid DNA
Recommended Reaction Volume
10 – 50 µL
