EffiStart™ One-Step Probe RT-qPCR Master Mix (Glycerol-Free) is a comprehensive one-step real-time reverse transcription-polymerase chain reaction (RT-qPCR) kit designed specifically for cDNA synthesis and real-time PCR, all within the same tube. This product is equipped with Hot Start Taq DNA Polymerase, making it apt for probe-based detection. Setting up a reaction is streamlined and straightforward, requiring only the addition of the RNA template, primers, and probes. Importantly, this master mix is devoid of ROX reference dye, an attribute that not only adds to its convenience but also significantly reduces the risk of cross-contamination. As a glycerol-free formulation, this product further embodies the innovation and precision sought by today's researchers and scientists.

1. Achieve RNA to cDNA to PCR amplification seamlessly, eliminating the need for separate step-by-step procedures.
2. The use of Hot Start Taq DNA Polymerase minimizes non-specific binding and enhances yield.
3. Our glycerol-free formulation is ideal for lyophilization, ensuring minimal batch-to-batch variation and compatibility with automated platforms.