Reporter gene assays are crucial for high-throughput screening and signaling studies, but firefly luciferase assays are limited by the high cost. Our team has developed the Dual-Structural Chromogenic Enzyme (DSCE) reporter system, featuring membrane-tethered and secreted enzyme forms. This system enables direct interaction with substrates on the cell surface and supernatant, eliminating the need for cell lysis and expensive substrates. DSCE offers low cost, high sensitivity, long half-life, and easy operation. It can be detected using a widely compatible ELISA reader, significantly reducing drug screening costs and enhancing drug development efficiency.

1. Accurate.
2. More convenient (less operative steps).
3. Lower cost.
4. More stable signal.
5. Widely applied by researcher due to the universality of monitoring device.