Introduction
The VEGA™ benchtop system builds on the advantages of PacBio's long-read sequencing technology, specifically designed for modern genomics research. It offers high accuracy (90% of bases achieving Q30+), complete genome integrity, and direct methylation detection, including 5mC detection at CpG sites in native DNA.
Each SMRT Cell is equipped with 100,000 ZMWs, tailored for efficient sequencing of single samples. With just one SMRT Cell, it can achieve 20x coverage of a human genome or assemble up to 384 microbial genomes of 2 Gb, significantly enhancing research efficiency.
The design of the VEGA™ system not only considers performance but also emphasizes user experience. Its user-friendly interface and streamlined workflow allow even novice researchers to quickly get started without extensive training. Additionally, the VEGA™ system supports various library preparation methods, providing flexibility and convenience for different experimental needs.eds.
Features / strengths
•Diverse Applications:
⓵Whole Genome Sequencing: The VEGA™ system enables high-coverage analysis of the human genome,
De novo assembly, and methylation sequencing, helping researchers gain deeper insights into genome
structure and function.
⓶Targeted Sequencing: It supports amplicon sequencing and target enrichment panels, particularly the
PureTarget™ application, suitable for cancer and genetic disease research. These technologies allow
researchers to precisely analyze gene regions associated with specific diseases while preserving
methylation features.
⓷RNA Sequencing: The VEGA™ system supports full-length RNA sequencing (Kinnex™), providing
accurate transcript information that aids researchers in exploring the complexity of gene expression and
detailed analysis of transcriptome variations.
⓸Microbiology Research: It facilitates random metagenomic analysis and assembly, including multi-
community analysis of 16S rRNA, enhancing understanding of microbial community structure and
function.
•Efficiency Improvements: The VEGA™ system integrates automated analysis capabilities, allowing sequencing results to be directly converted into standard BAM format, significantly reducing data processing time. Its optimized hardware design and streamlined workflow enable researchers to easily perform integrated analyses from library preparation to results, quickly obtaining high-quality data.
•Advanced Technology: The VEGA™ system utilizes the latest HiFi sequencing technology, offering longer read lengths and higher accuracy, making the analysis of complex genomes more feasible. This technology is particularly suited for applications requiring high precision and integrity, such as structural variant analysis and repeat sequence characterization.
•Flexibility and Compatibility: The VEGA™ system is compatible with existing HiFi library preparation and data analysis ecosystems, supporting various data analysis tools and platforms. This allows researchers to choose the most suitable analytical methods based on their specific needs.
Specification in detail
Read accuracy
99.9% accuracy (with up to 95% of bases achieving Q30+)
W × D × H
55.7 cm (21.9 in) × 69.5 cm (27.4 in) × 76.8 cm (30.2 in)
Supporting document
