EliteCell NK kit enables to obtain high purity of CD3-/CD56+ NK cells with high cytotoxic ability from both peripheral blood mononuclear cells (PBMCs) and cord blood mononuclear cells (CBMCs).

Performance :
*Suitable for MNC (mononuclear cells) from whole blood and cord blood source.
*70%-90% NK cells (CD3-CD56+) at Day 14.
*1.0- 2.0 x10^9 total cells (per L) at Day 14.
*80%-100% cytotoxicity at E/T ratio 5:1, better efficacy.
Advantage :
*No feeder cells required, safe for clinical application.
*No pre-coating required, easy to operate.
NK kit expansion and proliferation :
*After culturing for 14 days with EliteCell NK kit, high cell numbers were expanded from PBMC of four healthy individuals. (1-2 x109 cells at Day 14).
CCK-8 cytotoxicity assay :
*At E/T=2, NK cells showed 60-80%cytotoxicity capacity; At E/T=5, NK cells showed 80-100% cytotoxic capacity.
NK cell phenotyping :
*Flow cytometry analysis revealed that EliteCell NK kit induces 70%-90% positive NK cell(CD3-/CD56+) at Day 14.