Introduction
Features / strengths
Illumina has pioneered major advances in sequencing simplicity, flexibility, and platform performance. Sequencing output has grown over ten thousand-fold, from less than 1 gigabase to 16 terabases per run, while the number of reads has increased from millions to tens of billions. Experiments that once required complex workflows now use simple push-button sequencing.
Specification in detail
iSeq 100
Small Whole-Genome Sequencing (microbe, virus), Targeted Gene Sequencing (amplicon-based, gene panel), etc.
MiniSeq
Small Whole-Genome Sequencing (microbe, virus), Targeted Gene Sequencing (amplicon-based, gene panel), 16S Metagenomic Sequencing, etc.
MiSeq
Small Whole-Genome Sequencing (microbe, virus), Targeted Gene Expression Profiling, miRNA & Small RNA Analysis, DNA-Protein Interaction Analysis (ChIP-Seq), etc.
NextSeq 550
Exome & Large Panel Sequencing (enrichment-based), Single-Cell Profiling (scRNA-Seq, scDNA-Seq, oligo tagging assays), Methylation Sequencing, etc.
NextSeq 1000/2000
Transcriptome Sequencing (total RNA-Seq, mRNA-Seq, gene expression profiling), Metagenomic Profiling (shotgun metagenomics, metatranscriptomics), Cell-Free Sequencing & Liquid Biopsy Analysis, etc.
NovaSeq (6000/ X Series)
Large Whole-Genome Sequencing (human, plant, animal), Exome & Large Panel Sequencing (enrichment-based), Chromatin Analysis (ATAC-Seq, ChIP-Seq), etc.
